F(ab′)2 Preparation Kit

Source :	N/A
Application :	Fragmentation
Shipping :	The product is shipped at room temperature.
Storage :	Store at 2-8℃.
Note :	For research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals.

Background :	F(ab′)2 Preparation Kit enables efficient generation of F(ab′)2 from IgG. This kit uses Immobilized Pepsin, a nonspecific endopeptidase that is active only at acid pH and irreversibly denatured at neutral or alkaline pH. Pepsin digestion typically produces a F(ab′)2 fragment (~110kDa by SDS-PAGE under non-reducing conditions) and numerous small peptides of the Fc portion. The resulting F(ab′)2 fragment is composed of a pair of Fab′ units connected by two disulfide bonds. The Fc fragment is extensively degraded and can be separated from F(ab′)2 by dialysis, gel filtration or ion exchange chromatography.
Keywords :	F(ab)2
Synonyms :	F(ab')2 fragments Micro Preparation Kit; F(ab')2 Preparation Kit; F(ab')2 微型制备试剂盒; F(ab')2 制备试剂盒; Fragmentation; F(ab)2 制备试剂盒; Fab 制备试剂盒; Fab2 制备试剂盒; 抗体片段试剂盒